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Characterization of complex ganglioside phenotypes - MOBILion Systems | ASMS 2022

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Optimization of an LC-HRIM-MS platform for characterization of complex ganglioside phenotypes in a GBA mutant mouse model for Parkinson's disease.

Author(s)

Rachel Harris PhD - MOBILion Systems, Inc

Abstract

An assay using LC-HRIM-MS was developed to quantify gangliosides at the molecular species level in brains from wildtype (WT), D409V heterozygous (HET), and homozygous (HOM) GBA mutant mice which are a model system for Parkinson's disease.

Customer Intro
Problem
Solution
Conclusions
Abstract
  • Developed a 30 min LC-HRIM-MS method for in depth ganglioside characterization of mouse brain samples for minimal ion suppression and high throughput.
  • Separated isomeric gangliosides in the mobility dimension that were unresolved in the LC dimension.
  • Demonstrated flexibility of HRIM analysis for high throughput detection and quantitation of abundant gangliosides for of  ganglioside profile characterization.
  • Observed differences in ganglioside relative abundances among WT, HET, and HOM D409V GBA mutant mice.
Results
Characterization of complex ganglioside phenotypes - MOBILion Systems | ASMS 2022

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