Posters
Separation of select targeted isobaric and isomeric PTMs - MOBILion Systems | ASMS 2022
High-throughput Monitoring of Biotherapeutic Critical Quality Attributes with High-Resolution Ion Mobility-Mass Spectrometry
Author(s)
Shahadat Reza PhD - MOBILion Systems, Inc
Abstract
HRIM technology was used by itself in Flow Injection Analysis (FIA) and incorporated into a traditional LC-MS characterization workflow to perform rapid, targeted analysis of CQAs that are often difficult to separate or may not be detected with platform LC-MS methods.
Customer Intro
Problem
Solution
Conclusions
Abstract
- HRIM-MS separates target isomers native Asp and modified iso-Asp peptide pair in FIA analysis.
- LC-HRIM-MS allows for reproducible high-resolution separation of a target native Asp and modified iso-Asp peptide pair across multiple LC gradients ranging from 20-min to5-min.
- The MOBIE HRIM platform allows for separation of select targeted isobaric and isomeric PTMs such as deamidation and isomerization, allowing for rapid characterization of target PTMs and detection of potential isomers.
- Integration with Protein Metrics Data Processing Workflows (Byos for HRIM) allows for streamlined and efficient data processing of biopharmaceutical characterization data sets.
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