High Resolution Ion Mobility-Mass Spectrometry for Lipid Analyses

High Resolution Ion Mobility-Mass Spectrometry for Lipid Analyses

Lipids are extremely complex biomolecules that have several essential roles in biological processes. They have been shown to play roles in several diseases including Alzheimer's disease (AD), Parkinson's disease (PD), cardiovascular disease (CVD), and some cancers. Due to the high complexity of the lipidome and the vast number of isomers present, accurate identification of all lipid species present in a profile is extremely difficult. Additionally, monitoring of more than one lipid class at a time can be cumbersome. Here we demonstrate how HRIM-MS can be used as a high throughput alternative to liquid chromatography-mass spectrometry (LC-MS) for separation of isobaric and isomeric lipid species.