Effectiveness of HighResolution Ion Mobility for Complex Analyses

Ion mobility is a structurally selective separation strategy that is now routinely integrated with mass spectrometry (IM-MS) to improve peak capacity while simultaneously aiding in the separation of isobaric signals such as those encountered in complex chemical samples. Structures for lossless ion manipulation (SLIM) technology provides a modular architecture for developing tailored ion processing systems, including ion mobility spectrometers. This article evaluates the separation capabilities of a SLIM‑based high-resolution ion mobility spectrometer (HRIM) incorporating an extended (~13 m) separation path length design in order to determine benefits for scientists working with complex and challenging analyte classes such as glycans, peptides, and lipids.